Cadmium affects the glutathione/glutaredoxin system in germinating pea seeds.
Identifieur interne : 000953 ( Main/Exploration ); précédent : 000952; suivant : 000954Cadmium affects the glutathione/glutaredoxin system in germinating pea seeds.
Auteurs : Moêz Smiri [Tunisie] ; Abdelilah Chaoui ; Nicolas Rouhier ; Eric Gelhaye ; Jean-Pierre Jacquot ; Ezzedine El FerjaniSource :
- Biological trace element research [ 1559-0720 ] ; 2011.
Descripteurs français
- KwdFr :
- Cadmium (métabolisme), Cadmium (pharmacologie), Germination (MeSH), Glutarédoxines (métabolisme), Glutathion (métabolisme), Glutathione peroxidase (métabolisme), Glutathione reductase (métabolisme), Graines (croissance et développement), Graines (effets des médicaments et des substances chimiques), Graines (métabolisme), Isoformes de protéines (métabolisme), Pois (croissance et développement), Pois (effets des médicaments et des substances chimiques), Pois (métabolisme), Stress physiologique (MeSH), Thiols (métabolisme).
- MESH :
- croissance et développement : Graines, Pois.
- effets des médicaments et des substances chimiques : Graines, Pois.
- métabolisme : Cadmium, Glutarédoxines, Glutathion, Glutathione peroxidase, Glutathione reductase, Graines, Isoformes de protéines, Pois, Thiols.
- pharmacologie : Cadmium.
- Germination, Stress physiologique.
English descriptors
- KwdEn :
- Cadmium (metabolism), Cadmium (pharmacology), Germination (MeSH), Glutaredoxins (metabolism), Glutathione (metabolism), Glutathione Peroxidase (metabolism), Glutathione Reductase (metabolism), Peas (drug effects), Peas (growth & development), Peas (metabolism), Protein Isoforms (metabolism), Seeds (drug effects), Seeds (growth & development), Seeds (metabolism), Stress, Physiological (MeSH), Sulfhydryl Compounds (metabolism).
- MESH :
- chemical , metabolism : Cadmium, Glutaredoxins, Glutathione, Glutathione Peroxidase, Glutathione Reductase, Protein Isoforms, Sulfhydryl Compounds.
- chemical , pharmacology : Cadmium.
- drug effects : Peas, Seeds.
- growth & development : Peas, Seeds.
- metabolism : Peas, Seeds.
- Germination, Stress, Physiological.
Abstract
The aim of this work was to investigate the effects of cadmium (Cd) on thiol and especially glutathione (GSH)-dependent reactions (glutathione content, glutaredoxin (Grx) content and activity, "glutathione" peroxidase (Gpx) activity, and glutathione reductase (GR) activity) in germinating pea seeds. Under Cd stress conditions, the overall activity as well as more specifically the expression of Grx C4 and Grx S12 increased. On the contrary, when incubated with Cd ions in vitro, the disulfide reductase activity of both isoforms was drastically inhibited. In the case of Grx C4, this correlated with the formation of protein dimers of 28 kDa as evidenced by electrophoresis analysis. Oxidative stress also affected the GSH status, since Cd treatment provoked (1) a pronounced stimulation in Gpx (a thioredoxin-dependent enzyme in plants) expression and (2) a drastic decrease in GR activity. These results are discussed in relation with the known contribution of Grx system to the thiol status during the germination of Cd-poisoned pea seeds.
DOI: 10.1007/s12011-010-8749-3
PubMed: 20552295
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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sort="Gelhaye, Eric" uniqKey="Gelhaye E" first="Eric" last="Gelhaye">Eric Gelhaye</name></author><author><name sortKey="Jacquot, Jean Pierre" sort="Jacquot, Jean Pierre" uniqKey="Jacquot J" first="Jean-Pierre" last="Jacquot">Jean-Pierre Jacquot</name></author><author><name sortKey="El Ferjani, Ezzedine" sort="El Ferjani, Ezzedine" uniqKey="El Ferjani E" first="Ezzedine" last="El Ferjani">Ezzedine El Ferjani</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:20552295</idno><idno type="pmid">20552295</idno><idno type="doi">10.1007/s12011-010-8749-3</idno><idno type="wicri:Area/Main/Corpus">000A00</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000A00</idno><idno type="wicri:Area/Main/Curation">000A00</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000A00</idno><idno 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Rouhier</name></author><author><name sortKey="Gelhaye, Eric" sort="Gelhaye, Eric" uniqKey="Gelhaye E" first="Eric" last="Gelhaye">Eric Gelhaye</name></author><author><name sortKey="Jacquot, Jean Pierre" sort="Jacquot, Jean Pierre" uniqKey="Jacquot J" first="Jean-Pierre" last="Jacquot">Jean-Pierre Jacquot</name></author><author><name sortKey="El Ferjani, Ezzedine" sort="El Ferjani, Ezzedine" uniqKey="El Ferjani E" first="Ezzedine" last="El Ferjani">Ezzedine El Ferjani</name></author></analytic><series><title level="j">Biological trace element research</title><idno type="eISSN">1559-0720</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Cadmium (metabolism)</term><term>Cadmium (pharmacology)</term><term>Germination (MeSH)</term><term>Glutaredoxins (metabolism)</term><term>Glutathione (metabolism)</term><term>Glutathione Peroxidase (metabolism)</term><term>Glutathione Reductase (metabolism)</term><term>Peas (drug effects)</term><term>Peas (growth & development)</term><term>Peas (metabolism)</term><term>Protein Isoforms (metabolism)</term><term>Seeds (drug effects)</term><term>Seeds (growth & development)</term><term>Seeds (metabolism)</term><term>Stress, Physiological (MeSH)</term><term>Sulfhydryl Compounds (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Cadmium (métabolisme)</term><term>Cadmium (pharmacologie)</term><term>Germination (MeSH)</term><term>Glutarédoxines (métabolisme)</term><term>Glutathion (métabolisme)</term><term>Glutathione peroxidase (métabolisme)</term><term>Glutathione reductase (métabolisme)</term><term>Graines (croissance et développement)</term><term>Graines (effets des médicaments et des substances chimiques)</term><term>Graines (métabolisme)</term><term>Isoformes de protéines (métabolisme)</term><term>Pois (croissance et développement)</term><term>Pois (effets des médicaments et des substances chimiques)</term><term>Pois (métabolisme)</term><term>Stress physiologique (MeSH)</term><term>Thiols (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cadmium</term><term>Glutaredoxins</term><term>Glutathione</term><term>Glutathione Peroxidase</term><term>Glutathione Reductase</term><term>Protein Isoforms</term><term>Sulfhydryl Compounds</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Cadmium</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Graines</term><term>Pois</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Peas</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Graines</term><term>Pois</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Peas</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Peas</term><term>Seeds</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cadmium</term><term>Glutarédoxines</term><term>Glutathion</term><term>Glutathione peroxidase</term><term>Glutathione reductase</term><term>Graines</term><term>Isoformes de protéines</term><term>Pois</term><term>Thiols</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Cadmium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Germination</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Germination</term><term>Stress physiologique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The aim of this work was to investigate the effects of cadmium (Cd) on thiol and especially glutathione (GSH)-dependent reactions (glutathione content, glutaredoxin (Grx) content and activity, "glutathione" peroxidase (Gpx) activity, and glutathione reductase (GR) activity) in germinating pea seeds. Under Cd stress conditions, the overall activity as well as more specifically the expression of Grx C4 and Grx S12 increased. On the contrary, when incubated with Cd ions in vitro, the disulfide reductase activity of both isoforms was drastically inhibited. In the case of Grx C4, this correlated with the formation of protein dimers of 28 kDa as evidenced by electrophoresis analysis. Oxidative stress also affected the GSH status, since Cd treatment provoked (1) a pronounced stimulation in Gpx (a thioredoxin-dependent enzyme in plants) expression and (2) a drastic decrease in GR activity. These results are discussed in relation with the known contribution of Grx system to the thiol status during the germination of Cd-poisoned pea seeds.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20552295</PMID><DateCompleted><Year>2011</Year><Month>09</Month><Day>22</Day></DateCompleted><DateRevised><Year>2013</Year><Month>11</Month><Day>21</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1559-0720</ISSN><JournalIssue CitedMedium="Internet"><Volume>142</Volume><Issue>1</Issue><PubDate><Year>2011</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Biological trace element research</Title><ISOAbbreviation>Biol Trace Elem Res</ISOAbbreviation></Journal><ArticleTitle>Cadmium affects the glutathione/glutaredoxin system in germinating pea seeds.</ArticleTitle><Pagination><MedlinePgn>93-105</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1007/s12011-010-8749-3</ELocationID><Abstract><AbstractText>The aim of this work was to investigate the effects of cadmium (Cd) on thiol and especially glutathione (GSH)-dependent reactions (glutathione content, glutaredoxin (Grx) content and activity, "glutathione" peroxidase (Gpx) activity, and glutathione reductase (GR) activity) in germinating pea seeds. Under Cd stress conditions, the overall activity as well as more specifically the expression of Grx C4 and Grx S12 increased. On the contrary, when incubated with Cd ions in vitro, the disulfide reductase activity of both isoforms was drastically inhibited. In the case of Grx C4, this correlated with the formation of protein dimers of 28 kDa as evidenced by electrophoresis analysis. Oxidative stress also affected the GSH status, since Cd treatment provoked (1) a pronounced stimulation in Gpx (a thioredoxin-dependent enzyme in plants) expression and (2) a drastic decrease in GR activity. These results are discussed in relation with the known contribution of Grx system to the thiol status during the germination of Cd-poisoned pea seeds.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Smiri</LastName><ForeName>Moêz</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Bio-Physiologie Cellulaires, Faculté des Sciences de Bizerte, Zarzouna, Tunisia. moez.smiri@scbiol.uhp-nancy.fr</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Chaoui</LastName><ForeName>Abdelilah</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Rouhier</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials></Author><Author ValidYN="Y"><LastName>Gelhaye</LastName><ForeName>Eric</ForeName><Initials>E</Initials></Author><Author ValidYN="Y"><LastName>Jacquot</LastName><ForeName>Jean-Pierre</ForeName><Initials>JP</Initials></Author><Author ValidYN="Y"><LastName>El Ferjani</LastName><ForeName>Ezzedine</ForeName><Initials>E</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2010</Year><Month>06</Month><Day>16</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biol Trace Elem Res</MedlineTA><NlmUniqueID>7911509</NlmUniqueID><ISSNLinking>0163-4984</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D054477">Glutaredoxins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D020033">Protein Isoforms</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D013438">Sulfhydryl Compounds</NameOfSubstance></Chemical><Chemical><RegistryNumber>00BH33GNGH</RegistryNumber><NameOfSubstance UI="D002104">Cadmium</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.11.1.9</RegistryNumber><NameOfSubstance UI="D005979">Glutathione Peroxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.8.1.7</RegistryNumber><NameOfSubstance UI="D005980">Glutathione Reductase</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002104" MajorTopicYN="N">Cadmium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018525" MajorTopicYN="Y">Germination</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D054477" MajorTopicYN="N">Glutaredoxins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005979" MajorTopicYN="N">Glutathione Peroxidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D005980" MajorTopicYN="N">Glutathione Reductase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018532" MajorTopicYN="N">Peas</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020033" MajorTopicYN="N">Protein Isoforms</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="N">Stress, Physiological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013438" MajorTopicYN="N">Sulfhydryl Compounds</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2010</Year><Month>05</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2010</Year><Month>06</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>6</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>6</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>9</Month><Day>23</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20552295</ArticleId><ArticleId IdType="doi">10.1007/s12011-010-8749-3</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Tunisie</li></country></list><tree><noCountry><name sortKey="Chaoui, Abdelilah" sort="Chaoui, Abdelilah" uniqKey="Chaoui A" first="Abdelilah" last="Chaoui">Abdelilah Chaoui</name><name sortKey="El Ferjani, Ezzedine" sort="El Ferjani, Ezzedine" uniqKey="El Ferjani E" first="Ezzedine" last="El Ferjani">Ezzedine El Ferjani</name><name sortKey="Gelhaye, Eric" sort="Gelhaye, Eric" uniqKey="Gelhaye E" first="Eric" last="Gelhaye">Eric Gelhaye</name><name sortKey="Jacquot, Jean Pierre" sort="Jacquot, Jean Pierre" uniqKey="Jacquot J" first="Jean-Pierre" last="Jacquot">Jean-Pierre Jacquot</name><name sortKey="Rouhier, Nicolas" sort="Rouhier, Nicolas" uniqKey="Rouhier N" first="Nicolas" last="Rouhier">Nicolas Rouhier</name></noCountry><country name="Tunisie"><noRegion><name sortKey="Smiri, Moez" sort="Smiri, Moez" uniqKey="Smiri M" first="Moêz" last="Smiri">Moêz Smiri</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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